ICSI or Intra-cytoplasmic sperm injection is a procedure done during the process of IVF (In Vitro Fertilization). In this process sperms are injected directly in the eggs to ensure fertilization. The method of intra-cytoplasmic injection of sperm (ICSI) is an elaborate micromanipulation technique when the sperm is aspirated in to a thin sharp glass capillary and inserted in to an egg through its shell.

Indication for ICSI is the inability of the sperm to fertilize the egg spontaneously (low count, low motility), immunological causes of infertility, failing of classical IVF cycles, later age, low number of matured eggs, when cryo-preserved sperm is used or when the sperm is collected by MESA/TESE method, when donor’s eggs are used etc. This method has a very high success rate of fertilization. The patient has to indeed undergo the normal procedure of super-ovulation and egg retrieval and the partner has to provide the sperm. In the view of collected data we can say that by fertilization of the egg using this method is not increased risk of a child with congenital developmental defect when compared to spontaneous conception.